5EYM
MEK1 IN COMPLEX WITH BI 847325
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 78.760, 78.760, 223.412 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.210 - 2.700 |
| R-factor | 0.2468 |
| Rwork | 0.243 |
| R-free | 0.30770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eqc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.901 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.210 | 2.890 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.160 | 0.700 |
| Number of reflections | 20634 | |
| <I/σ(I)> | 5.5 | 1.1 |
| Completeness [%] | 97.2 | 97.4 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 18 % PEG 4000, 100 mM Tris, 300 mM NaCl |
