5EYA

TRIM25 RING domain in complex with Ubc13-Ub conjugate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	53.047, 75.779, 169.101
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.966 - 2.400
R-factor	0.1919
Rwork	0.189
R-free	0.22750
RMSD bond length	0.006
RMSD bond angle	1.098
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.179	0.066	0.743
Rmeas	0.191	0.070	0.821
Rpim	0.066	0.024	0.331
Total number of observations	220683
Number of reflections	27181
<I/σ(I)>	3.9
Completeness [%]	98.2	100	86.4
Redundancy	8.1	8.2	4.8
CC(1/2)		0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Li citrate, 20% PEG 3350