5E8J

Crystal structure of mRNA cap guanine-N7 methyltransferase in complex with RAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	49.147, 50.487, 84.579
Unit cell angles	90.06, 92.41, 115.41

Refinement procedure

Resolution	28.162 - 2.350
R-factor	0.2129
Rwork	0.210
R-free	0.26850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bgv
RMSD bond length	0.003
RMSD bond angle	0.790
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	84.485	28.162	2.480
High resolution limit [Å]	2.350	7.430	2.350
Rmerge		0.032	0.349
Rmeas	0.111
Rpim	0.079	0.032	0.349
Total number of observations	45164	1487	5607
Number of reflections	28355
<I/σ(I)>	6.6	15.1	2
Completeness [%]	92.8	89.9	87.1
Redundancy	1.6	1.7	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M MES pH 6.5, 30% PEG 4000