5DFZ

Structure of Vps34 complex II from S. cerevisiae.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.939270, 1.254380, 1.254860
Spacegroup name	P 21 21 2
Unit cell lengths	215.180, 226.840, 127.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.358 - 4.400
R-factor	0.3687
Rwork	0.368
R-free	0.37620
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.703
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.900	4.580
High resolution limit [Å]	4.400	4.400
Rmerge		4.820
Number of reflections	40224
<I/σ(I)>	21	1
Completeness [%]	99.8	98.8
Redundancy	32.2	15.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.1	290	300 mM sodium acetate pH 5.1, 3% 1,5- Diaminopentanedihydrochloride. grown at 290 K for 5 days