5BXV
eIF4E complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-03-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.607, 67.830, 79.084 |
| Unit cell angles | 90.00, 112.43, 90.00 |
Refinement procedure
| Resolution | 38.600 - 2.100 |
| R-factor | 0.1634 |
| Rwork | 0.162 |
| R-free | 0.19480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wkw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.303 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 37630 | |
| <I/σ(I)> | 21.8 | 2 |
| Completeness [%] | 96.9 | 92.4 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 298 | 0.1 M Tris-HCl, and 22 % polyethylene glycol 4000 |
