5AJA
Crystal structure of mandrill SAMHD1 (amino acid residues 1-114) bound to Vpx isolated from mandrill and human DCAF1 (amino acid residues 1058-1396)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-07 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 102.021, 102.021, 265.001 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.820 - 2.649 |
R-factor | 0.1761 |
Rwork | 0.173 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cc9 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.118 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.810 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.080 | 1.450 |
Number of reflections | 24555 | |
<I/σ(I)> | 20.7 | 1.63 |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 9.4 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.16 M TRISODIUM CITRATE-HCL PH 5.2, 4% PEG 6000 |