5A2L
Crystal structure of scFv-SM3 in complex with APD-CGalNAc-RP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.080, 35.190, 66.290 |
| Unit cell angles | 90.00, 103.01, 90.00 |
Refinement procedure
| Resolution | 64.590 - 1.790 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20447 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sm3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.528 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.590 | 1.880 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.110 | 0.600 |
| Number of reflections | 19905 | |
| <I/σ(I)> | 7.6 | 2.4 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
