4ZHN
Crystal Structure of AlkB T208A mutant protein in complex with Co(II), 2-oxoglutarate, and methylated trinucleotide T-meA-T
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97904 |
Spacegroup name | P 43 |
Unit cell lengths | 41.373, 41.373, 116.569 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.255 - 1.333 |
R-factor | 0.1192 |
Rwork | 0.118 |
R-free | 0.14720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fd8 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.160 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | COMO |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.350 |
High resolution limit [Å] | 1.330 | 3.610 | 1.330 |
Rmerge | 0.048 | 0.036 | 0.176 |
Rmeas | 0.053 | 0.041 | 0.195 |
Rpim | 0.023 | 0.018 | 0.084 |
Total number of observations | 246733 | ||
Number of reflections | 44362 | ||
<I/σ(I)> | 17.4 | ||
Completeness [%] | 98.8 | 95.2 | 80.3 |
Redundancy | 5.6 | 5.2 | 5.3 |
CC(1/2) | 0.997 | 0.972 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22-24% (w/v) PEG3350, 10% glycerol, 200 mM sodium formate |