4ZHN

Crystal Structure of AlkB T208A mutant protein in complex with Co(II), 2-oxoglutarate, and methylated trinucleotide T-meA-T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97904
Spacegroup name	P 43
Unit cell lengths	41.373, 41.373, 116.569
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.255 - 1.333
R-factor	0.1192
Rwork	0.118
R-free	0.14720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fd8
RMSD bond length	0.006
RMSD bond angle	1.160
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	COMO
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.350
High resolution limit [Å]	1.330	3.610	1.330
Rmerge	0.048	0.036	0.176
Rmeas	0.053	0.041	0.195
Rpim	0.023	0.018	0.084
Total number of observations	246733
Number of reflections	44362
<I/σ(I)>	17.4
Completeness [%]	98.8	95.2	80.3
Redundancy	5.6	5.2	5.3
CC(1/2)		0.997	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	22-24% (w/v) PEG3350, 10% glycerol, 200 mM sodium formate