4ZHN
Crystal Structure of AlkB T208A mutant protein in complex with Co(II), 2-oxoglutarate, and methylated trinucleotide T-meA-T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 43 |
| Unit cell lengths | 41.373, 41.373, 116.569 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.255 - 1.333 |
| R-factor | 0.1192 |
| Rwork | 0.118 |
| R-free | 0.14720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fd8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | COMO |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.350 |
| High resolution limit [Å] | 1.330 | 3.610 | 1.330 |
| Rmerge | 0.048 | 0.036 | 0.176 |
| Rmeas | 0.053 | 0.041 | 0.195 |
| Rpim | 0.023 | 0.018 | 0.084 |
| Total number of observations | 246733 | ||
| Number of reflections | 44362 | ||
| <I/σ(I)> | 17.4 | ||
| Completeness [%] | 98.8 | 95.2 | 80.3 |
| Redundancy | 5.6 | 5.2 | 5.3 |
| CC(1/2) | 0.997 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22-24% (w/v) PEG3350, 10% glycerol, 200 mM sodium formate |
