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4ZHN

Crystal Structure of AlkB T208A mutant protein in complex with Co(II), 2-oxoglutarate, and methylated trinucleotide T-meA-T

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1Bmethylated trinucleotide DNA T-meA-Tpolymer3891.71synthetic construct
2AAlpha-ketoglutarate-dependent dioxygenase AlkBpolymer21123580.91UniProt (P05050)
In PDB
Escherichia coli (strain K12)Alkylated DNA repair protein AlkB,DNA oxidative demethylase AlkB
3ACOBALT (II) IONnon-polymer58.91Chemie (CO)
4A2-OXOGLUTARIC ACIDnon-polymer146.11Chemie (AKG)
5waterwater18.0250Chemie (HOH)
Sequence modifications
A: 12 - 216 (UniProt: P05050)
PDBExternal DatabaseDetails
Met 11-expression tag
Ala 208Thr 208engineered mutation
Asn 217-expression tag
Leu 218-expression tag
Tyr 219-expression tag
Phe 220-expression tag
Gln 221-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains2
Total formula weight24472.6
Non-Polymers*Number of molecules2
Total formula weight205.0
All*Total formula weight24677.6
*Water molecules are not included.

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PDB entries from 2024-07-17

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