4ZBN

Non-helical DNA Triplex Forms a Unique Aptamer Scaffold for High Affinity Recognition of Nerve Growth Factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-01
Detector	ADSC QUANTUM 210
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	109.201, 60.708, 68.589
Unit cell angles	90.00, 95.57, 90.00

Refinement procedure

Resolution	68.200 - 2.447
R-factor	0.20447
Rwork	0.202
R-free	0.25697
RMSD bond length	0.013
RMSD bond angle	2.112
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.200	2.510
High resolution limit [Å]	2.447	2.447
Rmerge	0.038	0.303
Number of reflections	15532
<I/σ(I)>	23.3	2.03
Completeness [%]	98.2	84.1
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	16 % PEG 8000, 100 mM HEPES pH 6.0, 100 mM Magnesium Acetate and 20% glycerol