4Z32
Crystal Structure of the FERM-SH2 Domains of Jak2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.193, 188.742, 118.585 |
| Unit cell angles | 90.00, 113.87, 90.00 |
Refinement procedure
| Resolution | 42.954 - 3.040 |
| R-factor | 0.2582 |
| Rwork | 0.257 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4po6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.603 |
| Data reduction software | XDS (January 10, 2014) |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.980 | 3.190 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.075 | 0.534 |
| Number of reflections | 87780 | |
| <I/σ(I)> | 9.5 | 2 |
| Completeness [%] | 96.4 | 98.9 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Na Citrate, 11-13% PEG 3350, 1.05% 1-butanol, 5 mM TCEP |
