4Z32
Crystal Structure of the FERM-SH2 Domains of Jak2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 118.193, 188.742, 118.585 |
Unit cell angles | 90.00, 113.87, 90.00 |
Refinement procedure
Resolution | 42.954 - 3.040 |
R-factor | 0.2582 |
Rwork | 0.257 |
R-free | 0.27640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4po6 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.603 |
Data reduction software | XDS (January 10, 2014) |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER (2.5.5) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.980 | 3.190 |
High resolution limit [Å] | 3.040 | 3.040 |
Rmerge | 0.075 | 0.534 |
Number of reflections | 87780 | |
<I/σ(I)> | 9.5 | 2 |
Completeness [%] | 96.4 | 98.9 |
Redundancy | 2.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Na Citrate, 11-13% PEG 3350, 1.05% 1-butanol, 5 mM TCEP |