4Z31
Crystal structure of the RC3H2 ROQ domain in complex with stem-loop and double-stranded forms of RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.299, 174.734, 61.290 |
| Unit cell angles | 90.00, 114.12, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.2454 |
| Rwork | 0.245 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qik |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.094 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.083 | 0.039 | 0.830 |
| Rmeas | 0.090 | 0.043 | 0.913 |
| Rpim | 0.036 | 0.018 | 0.377 |
| Total number of observations | 236295 | ||
| Number of reflections | 39757 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 99.1 | 99.7 | 97.6 |
| Redundancy | 5.9 | 6.2 | 5.4 |
| CC(1/2) | 0.963 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | The protein was diluted to 6.7 mg/ml then mixed with IER3 RNA at a ratio 1:2 before crystallized. 17% PEG10000, 0.1 M Bis-Tris pH6.5, 5% Ethylene glycol |
