4YZM
Humanized Roco4 bound to LRRK2-In1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97889 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.030, 89.070, 177.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.430 - 3.000 |
| R-factor | 0.19982 |
| Rwork | 0.196 |
| R-free | 0.28190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f0f |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.943 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.430 | 3.500 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.366 | |
| Number of reflections | 13688 | |
| <I/σ(I)> | 10.62 | 4.36 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 100 mM 1,3-bis(tris (hydroxymethyl)methylamino)propane (pH 8.5), 200 mM Na/K tartrate, 11% (m/v) PEG 3350 |
