4YL6
Crystal structure of truncated cerebral cavernous malformation 2 C-terminal adaptor domain in complex with an internal helix of mitogen-activated protein kinase kinase kinase 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-04-05 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.210, 61.210, 68.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.780 - 2.100 |
R-factor | 0.2049 |
Rwork | 0.203 |
R-free | 0.23720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ykd |
RMSD bond length | 0.002 |
RMSD bond angle | 0.636 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.780 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.298 | |
Number of reflections | 8073 | |
<I/σ(I)> | 31.2 | 5.9 |
Completeness [%] | 99.4 | 96.4 |
Redundancy | 11 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 3.5 M Sodium Formate |