4YIG

vaccinia virus D4/A20(1-50) in complex with dsDNA containing an abasic site and free uracyl

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 65
Unit cell lengths	136.092, 136.092, 161.073
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.860 - 2.700
R-factor	0.18227
Rwork	0.180
R-free	0.22764
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4od8 1ssp
RMSD bond length	0.014
RMSD bond angle	1.820
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.860	2.790
High resolution limit [Å]	2.700	2.700
Rmerge		0.492
Number of reflections	46345
<I/σ(I)>	19.1	2.9
Completeness [%]	99.9	100
Redundancy	5	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	293	0.2M ammonium chloride, 10% W/V PEG 3350