4YIG

vaccinia virus D4/A20(1-50) in complex with dsDNA containing an abasic site and free uracyl

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I (A, E, I)	Uracil-DNA glycosylase	polymer	232	26729.5	3	UniProt (P20536)	Vaccinia virus (strain Copenhagen) (VACV)	UDG
2	B, F, J (B, F, J)	DNA polymerase processivity factor component A20	polymer	52	5777.6	3	UniProt (P20995)	Vaccinia virus
3	C, G, K (C, G, K)	DNA (5'-D(*CP*TP*GP*TP*(ORP)P*AP*TP*CP*TP*T)-3')	polymer	10	2892.9	3		synthetic construct
4	D, H, L (D, H, L)	DNA (5'-D(*AP*AP*GP*AP*TP*AP*AP*CP*AP*G)-3')	polymer	10	3086.1	3		synthetic construct
5	M, N, O (A, E, I)	URACIL	non-polymer		112.1	3	Chemie (URA)
6	P, Q, R, S, T... (A, B, C, E, F...)	water	water		18.0	54	Chemie (HOH)

Sequence modifications

A, E, I: 1 - 218 (UniProt: P20536)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

B, F, J: 3 - 50 (UniProt: P20995)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Met 1	-	expression tag
Ala 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	115458.0
Non-Polymers*	Number of molecules	3
	Total formula weight	336.3
All*	Total formula weight	115794.2

*Water molecules are not included.