4Y7R
Crystal structure of WDR5 in complex with MYC MbIIIb peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-29 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 80.762, 87.177, 44.914 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.589 - 1.898 |
R-factor | 0.1601 |
Rwork | 0.159 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xl2 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.958 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1951)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.589 | 1.930 |
High resolution limit [Å] | 1.898 | 1.900 |
Rmerge | 0.670 | |
Number of reflections | 25709 | |
<I/σ(I)> | 24.8 | 4.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.7 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M Tris 7.0, 25% PEG 8K |