4Y5Y

Diabody 330 complex with EpoR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-06
Detector	PSI PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 2 21 21
Unit cell lengths	78.141, 95.799, 144.972
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.146 - 2.850
Rwork	0.221
R-free	0.24080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s34 1eer
Data scaling software	Aimless (0.2.17)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.320	48.320	2.905
High resolution limit [Å]	2.850	9.010	2.850
Rmerge	0.100	0.026	0.727
Rpim	0.054	0.015	0.383
Total number of observations	110916	3289	16790
Number of reflections	25898
<I/σ(I)>	11.1	32.9	1.9
Completeness [%]	99.4	97.1	99.9
Redundancy	4.3	3.7	4.5
CC(1/2)	0.997	0.999	0.781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	Magnesium chloride, Tris, PEG 8000