4XST

Structure of the endoglycosidase-H treated L1-CR domains of the human insulin receptor in complex with residues 697-719 of the human insulin receptor (A-isoform)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 63 2 2
Unit cell lengths	158.718, 158.718, 85.912
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	79.360 - 3.000
R-factor	0.2191
Rwork	0.217
R-free	0.26220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hr7
RMSD bond length	0.010
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.360	6.000	3.100
High resolution limit [Å]	3.000	4.000	3.000
Rmerge	0.500	0.372	0.893
Rmeas		0.381	9.140
Number of reflections	13240	3959	1198
<I/σ(I)>	7.8	11.09	0.6
Completeness [%]	99.9	100	100
Redundancy	21.2		21.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2.45 M (NH4)2SO4 + 10 % glycerol