4XGZ
Crystal structure of human paxillin LD2 motif in complex with Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 108.189, 111.004, 143.865 |
| Unit cell angles | 94.65, 95.28, 114.53 |
Refinement procedure
| Resolution | 142.000 - 2.500 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pgf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS (3.3.20) |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.721 | |
| Number of reflections | 207767 | |
| <I/σ(I)> | 7.4 | 1.1 |
| Completeness [%] | 94.3 | 94.2 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.2 M sodium nitrate |
