4XDK
Crystal structure of human two pore domain potassium ion channel TREK2 (K2P10.1) in complex with norfluoxetine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.190, 113.030, 112.500 |
| Unit cell angles | 90.00, 90.41, 90.00 |
Refinement procedure
| Resolution | 39.870 - 3.600 |
| R-factor | 0.2479 |
| Rwork | 0.248 |
| R-free | 0.25410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xdj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.910 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.870 | 3.690 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.045 | 0.808 |
| Number of reflections | 21872 | |
| <I/σ(I)> | 15.1 | 1.8 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2M KCl, 0.2M ammonium formate, 0.1M Tris pH 7, 31% pentaerythritol ethoxylate 15/04 |
