4XDJ
Crystal structure of human two pore domain potassium ion channel TREK2 (K2P10.1) in an alternate conformation (FORM 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.745, 113.871, 111.782 |
| Unit cell angles | 90.00, 90.97, 90.00 |
Refinement procedure
| Resolution | 37.260 - 3.800 |
| R-factor | 0.2637 |
| Rwork | 0.263 |
| R-free | 0.27690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bw5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.370 | 3.900 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.070 | 0.816 |
| Number of reflections | 18910 | |
| <I/σ(I)> | 10.8 | 1.8 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2M KCl, 0.1M sodium cacodylate pH6.5, 22% (w/v) PEG1500 + 3% (v/v) Methanol |
