4XDJ
Crystal structure of human two pore domain potassium ion channel TREK2 (K2P10.1) in an alternate conformation (FORM 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.745, 113.871, 111.782 |
Unit cell angles | 90.00, 90.97, 90.00 |
Refinement procedure
Resolution | 37.260 - 3.800 |
R-factor | 0.2637 |
Rwork | 0.263 |
R-free | 0.27690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bw5 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.370 | 3.900 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.070 | 0.816 |
Number of reflections | 18910 | |
<I/σ(I)> | 10.8 | 1.8 |
Completeness [%] | 99.0 | 99.5 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2M KCl, 0.1M sodium cacodylate pH6.5, 22% (w/v) PEG1500 + 3% (v/v) Methanol |