4XBF

Structure of LSD1:CoREST in complex with ssRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	I 2 2 2
Unit cell lengths	121.073, 178.411, 234.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.018 - 2.803
R-factor	0.202
Rwork	0.201
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2iw5
RMSD bond length	0.003
RMSD bond angle	0.743
Data reduction software	HKL-2000 (Version v706)
Refinement software	PHENIX ((phenix.refine: dev_1839))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.018	2.873
High resolution limit [Å]	2.803	2.803
Number of reflections	62270
<I/σ(I)>	23.8
Completeness [%]	99.5	96.8
Redundancy	14.6	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	290	The LSD1-CoREST complex 10-12 mg/ml concentration (in 25 mM HEPES-Na, pH 7.4, 100 mM NaCl, 5 mM DTT, 1 mM PMSF) was mixed (1:1) with the reservoir solution [0.60 M Li2SO4, 0.63 M (NH4)2SO4, 0.25 M NaCl, 100 mM Na-citrate, pH 5.6, 10 mM DTT]