4XBF
Structure of LSD1:CoREST in complex with ssRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 121.073, 178.411, 234.676 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.018 - 2.803 |
R-factor | 0.202 |
Rwork | 0.201 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2iw5 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.743 |
Data reduction software | HKL-2000 (Version v706) |
Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.018 | 2.873 |
High resolution limit [Å] | 2.803 | 2.803 |
Number of reflections | 62270 | |
<I/σ(I)> | 23.8 | |
Completeness [%] | 99.5 | 96.8 |
Redundancy | 14.6 | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 290 | The LSD1-CoREST complex 10-12 mg/ml concentration (in 25 mM HEPES-Na, pH 7.4, 100 mM NaCl, 5 mM DTT, 1 mM PMSF) was mixed (1:1) with the reservoir solution [0.60 M Li2SO4, 0.63 M (NH4)2SO4, 0.25 M NaCl, 100 mM Na-citrate, pH 5.6, 10 mM DTT] |