4WZI
Crystal structure of crosslink stabilized long-form PDE4B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 137.830, 137.830, 141.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.010 - 2.580 |
R-factor | 0.1737 |
Rwork | 0.172 |
R-free | 0.20570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g45 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.010 | 2.590 |
High resolution limit [Å] | 2.580 | 2.580 |
Rmerge | 0.417 | |
Number of reflections | 48625 | |
<I/σ(I)> | 18.5 | 2.1 |
Completeness [%] | 98.6 | 56.2 |
Redundancy | 4.8 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 21% PEG 3350, 0.1 M potassium iodide, 0.1 M HEPES |