4WZI
Crystal structure of crosslink stabilized long-form PDE4B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 137.830, 137.830, 141.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.010 - 2.580 |
| R-factor | 0.1737 |
| Rwork | 0.172 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g45 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.010 | 2.590 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.417 | |
| Number of reflections | 48625 | |
| <I/σ(I)> | 18.5 | 2.1 |
| Completeness [%] | 98.6 | 56.2 |
| Redundancy | 4.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 21% PEG 3350, 0.1 M potassium iodide, 0.1 M HEPES |
