4WXS

Crystal Structure of the E396D SNP Variant of the Myocilin Olfactomedin Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2013-04-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	I 2 2 2
Unit cell lengths	68.880, 86.650, 87.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.790 - 1.900
R-factor	0.1757
Rwork	0.172
R-free	0.20980
RMSD bond length	0.003
RMSD bond angle	0.766
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.790
High resolution limit [Å]	1.900
Number of reflections	130265
<I/σ(I)>	15.52
Completeness [%]	100.0
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	Peg 3000 CAPSO Peg 400