4WXG
Crystal structure of L-Serine Hydroxymethyltransferase in complex with a mixture of L-Threonine and Glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-06-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 115.430, 115.430, 191.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.430 - 2.000 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wxb |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.014 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.088 | 0.672 |
| Number of reflections | 82377 | 24758 |
| <I/σ(I)> | 14.4 | 2.2 |
| Completeness [%] | 99.9 | 96.8 |
| Redundancy | 8.1 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | HEPES 20 mM pH 7.5, Sodium citrate 1.1 M, Cacodylate 0.1 M pH 6,5 |
