4WV6

Heterodimer of Importin alpha 1 with nuclear localization signal of TAF8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-07
Detector	ADSC QUANTUM 210
Wavelength(s)	0.93340
Spacegroup name	P 21 21 21
Unit cell lengths	54.270, 77.720, 128.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.536 - 1.750
R-factor	0.1546
Rwork	0.153
R-free	0.17990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rz9
RMSD bond length	0.008
RMSD bond angle	1.158
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.540	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.050	0.774
Number of reflections	55423
<I/σ(I)>	19.18	2.03
Completeness [%]	99.6	99.14
Redundancy	4.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	281.15	HEPES-NaOH, polyethylene glycole 3350, L-proline