4WUA
Crystal structure of human SRPK1 complexed to an inhibitor SRPIN340
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 75.132, 75.132, 310.623 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.870 - 2.000 |
| R-factor | 0.19861 |
| Rwork | 0.197 |
| R-free | 0.23634 |
| Starting model (for MR) | 1wak |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.848 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 36614 | |
| <I/σ(I)> | 71.03 | |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 20.9 | 21.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M sodium citrate, 0.2M ammonium acetate, 5%PEG6000 |
