4WRL

Structure of the human CSF-1:CSF-1R complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-25
Detector	PSI PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	I 41
Unit cell lengths	142.997, 142.997, 138.323
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.710 - 2.802
R-factor	0.225
Rwork	0.223
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dkd 3uf2
RMSD bond length	0.006
RMSD bond angle	1.094
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.710	2.970
High resolution limit [Å]	2.800	2.800
Rmerge	0.064	0.815
Number of reflections	34125
<I/σ(I)>	20.73	2.32
Completeness [%]	99.8	99.2
Redundancy	6.9	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5 and 28% w/v PEG 3350