4WRL

Structure of the human CSF-1:CSF-1R complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	Macrophage colony-stimulating factor 1 receptor	polymer	286	31583.9	2	UniProt (P07333) Pfam (PF00047)	Homo sapiens (Human)	CSF-1 receptor,M-CSF-R,Proto-oncogene c-Fms
2	C, D (B, D)	Macrophage colony-stimulating factor 1	polymer	170	19757.3	2	UniProt (P09603) Pfam (PF05337)	Homo sapiens (Human)	MCSF,Lanimostim
3	E, H (E, H)	N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		820.7	2	In PDB GlyTouCan (G43476MN)
4	F (F)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		383.3	1	In PDB GlyTouCan (G00055MO)
5	G, I (G, I)	N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		674.6	2	In PDB BIRD (PRD_900067) GlyTouCan (G00065MO)		3'-sialyl-N-acetyllactosamine
6	J (A)	N-acetyl-alpha-neuraminic acid	non-polymer		309.3	1	Chemie (SIA)
7	K, L, M (A, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)

Sequence modifications

A, C: 20 - 296 (UniProt: P07333)

PDB	External Database	Details
Gln 240	Asn 240	engineered mutation
Gly 297	-	expression tag
Thr 298	-	expression tag
Lys 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag
His 305	-	expression tag

B, D: 1 - 149 (UniProt: P09603)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	102682.4
Branched	Number of molecules	5
	Total formula weight	3374.0
Non-Polymers*	Number of molecules	4
	Total formula weight	972.9
All*	Total formula weight	107029.3

*Water molecules are not included.