4WO1
Crystal structure of the DAP12 transmembrane domain in lipid cubic phase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 50.797, 43.685, 50.605 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.121 - 2.140 |
R-factor | 0.2382 |
Rwork | 0.232 |
R-free | 0.29570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wol |
RMSD bond length | 0.007 |
RMSD bond angle | 0.982 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.120 |
High resolution limit [Å] | 2.140 |
Rmerge | 0.137 |
Number of reflections | 6316 |
<I/σ(I)> | 15.46 |
Completeness [%] | 95.7 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.07 | 293 | 0.1 M bis-tris propane chloride pH 6.07, 19.7 %w/v PEG 3350, 0.27 M calcium chloride |