4WO1

Crystal structure of the DAP12 transmembrane domain in lipid cubic phase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 2
Unit cell lengths	50.797, 43.685, 50.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.121 - 2.140
R-factor	0.2382
Rwork	0.232
R-free	0.29570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wol
RMSD bond length	0.007
RMSD bond angle	0.982
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	33.120
High resolution limit [Å]	2.140
Rmerge	0.137
Number of reflections	6316
<I/σ(I)>	15.46
Completeness [%]	95.7
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.07	293	0.1 M bis-tris propane chloride pH 6.07, 19.7 %w/v PEG 3350, 0.27 M calcium chloride