4WFN
Crystal structure of the large ribosomal subunit (50S) of Deinococcus radiodurans containing a three residue insertion in L22 in complex with erythromycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 85 |
Detector technology | CCD |
Collection date | 2012-09-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 170.092, 411.588, 695.883 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.988 - 3.540 |
R-factor | 0.2364 |
Rwork | 0.234 |
R-free | 0.28230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zjr |
RMSD bond length | 0.007 |
RMSD bond angle | 1.246 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.600 |
High resolution limit [Å] | 3.540 | 3.540 |
Rmerge | 0.164 | 0.818 |
Number of reflections | 270450 | |
<I/σ(I)> | 8.3 | 1.57 |
Completeness [%] | 91.0 | 90.3 |
Redundancy | 5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | Magnesuim chloride, Hepes, ammonium chloride, ethanol, 2-ethyl-1,3-hexadiol |