4WFH

Human TRAAK K+ channel in a Tl+ bound nonconductive conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9781
Spacegroup name	P 1 21 1
Unit cell lengths	80.972, 138.755, 96.759
Unit cell angles	90.00, 94.64, 90.00

Refinement procedure

Resolution	48.200 - 3.010
R-factor	0.20502
Rwork	0.203
R-free	0.23850
RMSD bond length	0.006
RMSD bond angle	0.908
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	3.050
High resolution limit [Å]	3.000	3.000
Number of reflections	39746
<I/σ(I)>	11.3	0.6
Completeness [%]	100.0	100
Redundancy	10.5	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	277	50 MM TRIS PH 8.8, 200 MM CA(NO3)2, 27-30% (VOL/VOL) PEG400