4UV7
The complex structure of extracellular domain of EGFR and GC1118A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 95 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.389, 84.172, 97.793 |
Unit cell angles | 90.00, 97.11, 90.00 |
Refinement procedure
Resolution | 19.750 - 2.100 |
R-factor | 0.20429 |
Rwork | 0.202 |
R-free | 0.24759 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yy9 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.535 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.900 | 1.870 |
Rmerge | 0.090 | 0.940 |
Number of reflections | 104241 | |
<I/σ(I)> | 19.38 | 1.2 |
Completeness [%] | 98.1 | 84.6 |
Redundancy | 6.9 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |