4UP5
Crystal structure of the Pygo2 PHD finger in complex with the B9L HD1 domain and a chemical fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 54.270, 54.270, 57.655 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.520 - 1.650 |
| R-factor | 0.18333 |
| Rwork | 0.182 |
| R-free | 0.21117 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xb1 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.097 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.950 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.100 | 1.410 |
| Number of reflections | 10894 | |
| <I/σ(I)> | 16.7 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 60% TACSIMATE, PH 7. |
