4UP5

Crystal structure of the Pygo2 PHD finger in complex with the B9L HD1 domain and a chemical fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-10-12
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 41 21 2
Unit cell lengths	54.270, 54.270, 57.655
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.520 - 1.650
R-factor	0.18333
Rwork	0.182
R-free	0.21117
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xb1
RMSD bond length	0.022
RMSD bond angle	2.097
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.7.0002)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.950	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.100	1.410
Number of reflections	10894
<I/σ(I)>	16.7	2.1
Completeness [%]	100.0	100
Redundancy	13	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				60% TACSIMATE, PH 7.