4UOV
The structure of a tetrameric alpha-carbonic anhydrase from Thermovibrio ammonificans reveals a core formed around intermolecular disulfides, which contribute to its thermostability.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-10 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 163.750, 283.020, 52.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.010 - 1.850 |
| R-factor | 0.17341 |
| Rwork | 0.172 |
| R-free | 0.20404 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c3t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.266 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.010 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.100 | 0.950 |
| Number of reflections | 206954 | |
| <I/σ(I)> | 13.7 | 2.4 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 9% ETHYLENE GLYCOL, 9 % PEG8000, 10 MM CARBOXYLIC ACIDS ADDITIVE MIXTURE, 100MM MES PH 6.5. |
