4UEL
UCH-L5 in complex with ubiquitin-propargyl bound to the RPN13 DEUBAD domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-02 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.340, 98.600, 100.211 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.280 - 2.300 |
| R-factor | 0.19365 |
| Rwork | 0.191 |
| R-free | 0.23426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.993 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.340 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.130 | 0.830 |
| Number of reflections | 26979 | |
| <I/σ(I)> | 9.7 | 1.6 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | 100 MM BIS-TRIS-PROPANE PH 5.8, 300 MM NABR, 21% PEG3350. 4 DEGREES CELSIUS |
