4UDC
GR in complex with dexamethasone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.660, 84.660, 105.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.810 - 2.500 |
| R-factor | 0.2121 |
| Rwork | 0.210 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m2z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.800 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.550 |
| Number of reflections | 15574 | |
| <I/σ(I)> | 8.8 | 1.6 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10% PEG8000, 10% ETHYLENE GLYCOL, 0.1 M HEPES PH 7.5 |
