4U7T

Crystal structure of DNMT3A-DNMT3L in complex with histone H3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 65
Unit cell lengths	183.823, 183.823, 123.265
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.213 - 2.900
R-factor	0.225
Rwork	0.223
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a1a 2qrv
RMSD bond length	0.010
RMSD bond angle	1.408
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.900
Rmerge	0.095
Number of reflections	52477
<I/σ(I)>	139.3
Completeness [%]	99.9
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Sodium Acetate, 0.6M Ammonium Sulfate