4U3J

TOG2:alpha/beta-tubulin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	111.909, 89.573, 135.508
Unit cell angles	90.00, 112.31, 90.00

Refinement procedure

Resolution	34.069 - 2.810
R-factor	0.2197
Rwork	0.218
R-free	0.25880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ffb 2qk1
RMSD bond length	0.003
RMSD bond angle	0.656
Refinement software	PHENIX ((phenix.refine: dev_1627))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.920
High resolution limit [Å]	2.810	2.810
Rmerge	0.143	0.924
Number of reflections	26235
<I/σ(I)>	9.6	1.1
Completeness [%]	86.5	35.3
Redundancy	4.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	298	25% (v/v) PEG3350, 0.25 M (NH4)2SO4, 0.1 M MES