4U1G
Plasmodium falciparum reticulocyte-binding protein homologue 5 (PfRH5) bound to monoclonal antibody QA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-14 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 65.120, 137.300, 228.579 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.240 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.049 | 0.380 |
Number of reflections | 36313 | |
<I/σ(I)> | 9.2 | 2.3 |
Completeness [%] | 95.5 | 96.3 |
Redundancy | 4.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.12 M mix of 1,6-hexanediol, 1-butanol, 1,2-propanediol, 2-propanol, 1,4-butanediol, 1,3-propanediol. 0.1 M MES-imidazole (pH 6.5) 20% (w/v) PEG 550 MM 10% (w/v) PEG 20,000 |