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2ZN9

Crystal structure of Ca2+-bound form of des3-20ALG-2

2ZN9 の概要
エントリーDOI10.2210/pdb2zn9/pdb
関連するPDBエントリー2ZN8 2ZND 2ZNE
分子名称Programmed cell death protein 6, CALCIUM ION, NONAETHYLENE GLYCOL, ... (5 entities in total)
機能のキーワードpenta-ef-hand protein, calcium binding protein, apoptosis, endoplasmic reticulum, membrane, nucleus, polymorphism
由来する生物種Homo sapiens (Human)
細胞内の位置Nucleus membrane; Peripheral membrane protein: O75340
タンパク質・核酸の鎖数2
化学式量合計41558.66
構造登録者
Suzuki, H.,Kawasaki, M.,Inuzuka, T.,Kakiuchi, T.,Shibata, H.,Wakatsuki, S.,Maki, M. (登録日: 2008-04-22, 公開日: 2008-09-09, 最終更新日: 2023-11-01)
主引用文献Suzuki, H.,Kawasaki, M.,Inuzuka, T.,Okumura, M.,Kakiuchi, T.,Shibata, H.,Wakatsuki, S.,Maki, M.
Structural Basis for Ca(2+)-Dependent Formation of ALG-2/Alix Peptide Complex: Ca(2+)/EF3-Driven Arginine Switch Mechanism
Structure, 16:1562-1573, 2008
Cited by
PubMed Abstract: ALG-2 belongs to the penta-EF-hand (PEF) protein family and interacts with various intracellular proteins, such as Alix and TSG101, that are involved in endosomal sorting and HIV budding. Through X-ray crystallography, we solved the structures of Ca(2+)-free and -bound forms of N-terminally truncated human ALG-2 (des3-20ALG-2), Zn(2+)-bound form of full-length ALG-2, and the structure of the complex between des3-23ALG-2 and the peptide corresponding to Alix799-814 in Zn(2+)-bound form. Binding of Ca(2+) to EF3 enables the side chain of Arg125, present in the loop connecting EF3 and EF4, to move enough to make a primary hydrophobic pocket accessible to the critical PPYP motif, which partially overlaps with the GPP motif for the binding of Cep55 (centrosome protein 55 kDa). Based on these results, together with the results of in vitro binding assay with mutant ALG-2 and Alix proteins, we propose a Ca(2+)/EF3-driven arginine switch mechanism for ALG-2 binding to Alix.
PubMed: 18940611
DOI: 10.1016/j.str.2008.07.012
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.4 Å)
構造検証レポート
Validation report summary of 2zn9
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-04-02に公開中

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