4S1T
Crystal structure of the mutant I26A/N52A of the endoribonuclease from human coronavirus 229E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 86.445, 140.285, 426.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.222 - 2.504 |
| R-factor | 0.2431 |
| Rwork | 0.241 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rs4 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.514 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.429 | |
| Number of reflections | 89338 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2M sodium citrate tribasic dehydrate, pH 8.5, 15% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
