4S10
Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2011 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.598, 46.334, 76.438 |
| Unit cell angles | 103.00, 92.03, 89.85 |
Refinement procedure
| Resolution | 19.722 - 2.614 |
| R-factor | 0.2275 |
| Rwork | 0.223 |
| R-free | 0.31250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.402 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.720 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.074 | 13.600 |
| Number of reflections | 14057 | |
| <I/σ(I)> | 8.8 | 5.7 |
| Completeness [%] | 93.1 | 91.5 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 288 | 30% PEG 1500, VAPOR DIFFUSION, temperature 288K |
