4RSU
Crystal structure of the light and hvem complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-05 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.651, 113.604, 163.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.920 - 2.300 |
| R-factor | 0.19052 |
| Rwork | 0.188 |
| R-free | 0.23145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kg8 4fhq |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.081 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.125 | 0.936 |
| Number of reflections | 92792 | |
| <I/σ(I)> | 21.7 | 3.1 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 10.8 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 290 | 0.2M MAGNESIUM CHLORIDE, 0.1M BIS:TRIS BUFFER, 9% W/V PEG3350, PH 5.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290.0K |
