4RNO
Crystal structure of human polymerase eta extending an abasic site-dA pair by inserting dCTP opposite template G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.07810 |
Spacegroup name | P 61 |
Unit cell lengths | 98.788, 98.788, 82.008 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.776 - 2.820 |
R-factor | 0.2431 |
Rwork | 0.241 |
R-free | 0.28160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o3n |
RMSD bond length | 0.014 |
RMSD bond angle | 0.648 |
Refinement software | PHENIX ((phenix.refine: dev_1810)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.720 | 2.900 |
High resolution limit [Å] | 2.820 | 3.590 | 2.850 |
Rmerge | 0.207 | ||
Number of reflections | 10375 | ||
<I/σ(I)> | 5.025 | ||
Completeness [%] | 94.0 | 98.9 | 58.6 |
Redundancy | 5 | 5.4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M MES pH 5.5, 5mM magnesium chloride, 28% PEG 2000 MME , VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |