4REB
Structural Insights into 5' Flap DNA Unwinding and Incision by the Human FAN1 Dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 31 |
Unit cell lengths | 104.770, 104.770, 127.550 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 90.700 - 4.200 |
R-factor | 0.2321 |
Rwork | 0.230 |
R-free | 0.26210 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.511 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.700 | 4.310 |
High resolution limit [Å] | 4.200 | 4.200 |
Rmerge | 0.076 | |
Number of reflections | 10793 | |
<I/σ(I)> | 7.8 | |
Completeness [%] | 99.3 | 99.88 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | micro-batch under oil | 300 | 0.1mM spermidine, 1% 1,6-Hexanediol, 22-24% PEG 3350, 0.1M BisTris Propane, micro-batch under oil, temperature 300K |