4R67
Human constitutive 20S proteasome in complex with carfilzomib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2013-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 171.989, 201.027, 225.585 |
| Unit cell angles | 90.00, 107.93, 90.00 |
Refinement procedure
| Resolution | 33.505 - 2.890 |
| R-factor | 0.2166 |
| Rwork | 0.215 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.712 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX ((Phaser-MR)) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.990 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Number of reflections | 320820 | |
| Completeness [%] | 98.7 | 91.7 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.2M sodium formate, 40% MPD, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
