4R26

Crystal structure of human Fab PGT124, a broadly neutralizing and potent HIV-1 neutralizing antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 1 21 1
Unit cell lengths	62.686, 40.331, 84.457
Unit cell angles	90.00, 91.67, 90.00

Refinement procedure

Resolution	42.211 - 2.497
R-factor	0.235
Rwork	0.233
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.565
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((molecular replacement))
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.211
High resolution limit [Å]	2.497
Number of reflections	14303
Completeness [%]	95.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	20% PEG 4000, 0.2M MgCl2, 0.1M Tris-HCL, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K