4QMG

The Structure of MTDH-SND1 Complex Reveals Novel Cancer-Promoting Interactions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97849
Spacegroup name	P 1 21 1
Unit cell lengths	121.830, 65.715, 135.841
Unit cell angles	90.00, 96.31, 90.00

Refinement procedure

Resolution	45.071 - 2.701
R-factor	0.1917
Rwork	0.190
R-free	0.24760
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.723
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	59029
<I/σ(I)>	20.4	2.65
Completeness [%]	100.0	100
Redundancy	7.3	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	296	Mix 275nl of 15mg/ml SeMet-SND1(16-339)-L21-MTDH(386-407) with 250nl of well buffer (21.6% pEG3350, 0.1M Sodium Citrate, PH8.0, 0.1M CsCl )+50nl of micro seeds., VAPOR DIFFUSION, SITTING DROP, temperature 296K